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william’s e medium glutamax  (Thermo Fisher)


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    Thermo Fisher william’s e medium glutamax
    William’s E Medium Glutamax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/william%E2%80%99s+medium+e/glutamax+medium/bio_rxiv__2025__07__21__665898-266-10-15
    Average 90 stars, based on 1 article reviews
    william’s e medium glutamax - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: METTL3 and FTO Regulate Heat Stress Response in Hu Sheep Through Lipid Metabolism via m6A Modification
    Article Snippet: For lipid deposition, primary hepatocytes were incubated with a 1.2 mM fatty acid solution [oleic acid (OA, Sigma-Aldrich): palmitic acid (PA, Sigma-Aldrich) = 2:1] in William’s Medium E (Gibco) containing 15% FBS (Gibco) for 24 h ( n = 3, three technical repetitions were performed for each sample).

    Article Title: Limited impact of hepatitis A virus 3C protease-mediated cleavage on the functions of NEMO in human hepatocytes.
    Article Snippet: PHH was purchased from BioIVT and cultured in William’s Medium E (Gibco), supplemented with 1% (v/v) P-S (Gibco), 1% (v/v) NAA (Gibco), 1% (v/v) glutamax (Gibco), 0.2% (v/v) normocin (Invivogen), 2% (v/v) B27 (Gibco), 1% (v/v) N2 supplement (Gibco), 100 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 μM Y27632 (Peprotech), 1 μM A83-01 (Tocris) and cultured at 37°C with 5% CO2.

    Article Title: Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
    Article Snippet: Cells were seeded at a density of 2.7 × 10 4 cells/cm 2 in culture flasks and were cultured in William’s Medium E (Gibco/Thermo Fisher Scientific, Darmstadt, Germany) containing 10% FCS, 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 μg/mL insulin (Merck), 50 μM hydrocortisone-hemisuccinate (Biomol, Hamburg, Germany) and 1X AAS.

    Article Title: The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria.
    Article Snippet: Livers were then perfused with William’s medium E (Gibco) and 40U/L of collagenase IV (Worthington Biochemical Corporation).

    Article Title: Salsalate improves the anti-tumor efficacy of lenvatinib in MASH-driven hepatocellular carcinoma
    Article Snippet: Cells were allowed to adhere overnight, and all experiments were performed in William’s Medium E (Gibco-Thermo Fisher Scientific, Waltham, MA, USA, Cat # A12176-01) supplemented with 10% FBS (unless serum starved), 1% antibiotic–antimycotic solution, and 1% L-glutamine.

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: PHH were resuspended in cooled William’s Medium E (Gibco, Thermo Fisher Scientific, cat. no. 32551087), supplemented with 10% FBS (Sigma-Aldrich, USA, cat. no. S0615-500ml), 15 mM HEPES (cat. no. 15630–056), 1 mM sodium pyruvate (cat. no. 11360– 039), 1% Penicillin/Streptomycin (cat. no. 15140122), 1% MEM NEAA (cat. no. 11140– 035, all provided by Gibco, Thermo Fisher Scientific, USA), 1 μg/mL Dexamethasone (Jenapharm/MIBE GmbH, Germany, cat. no. PZN: 08704404), and 32 U/L human insulin (Eli Lilly, Germany, cat. no. PZN: 02526396).

    Article Title: Discovery of the Clinical Candidate S-892216 : A Second-Generation of SARS-CoV-2 3CL Protease Inhibitor for Treating COVID-19.
    Article Snippet: Regarding liver microsomes, the assays were conducted as previously reported.25 Regarding hepatocytes, the compounds were incubated with 1.0 × 106 cells/ mL in suspension in the William’s Medium E (Thermo Fisher Scientific Inc.) at 37 °C.

    Suspension:

    Article Title: METTL3 and FTO Regulate Heat Stress Response in Hu Sheep Through Lipid Metabolism via m6A Modification
    Article Snippet: For lipid deposition, primary hepatocytes were incubated with a 1.2 mM fatty acid solution [oleic acid (OA, Sigma-Aldrich): palmitic acid (PA, Sigma-Aldrich) = 2:1] in William’s Medium E (Gibco) containing 15% FBS (Gibco) for 24 h ( n = 3, three technical repetitions were performed for each sample).

    Article Title: Limited impact of hepatitis A virus 3C protease-mediated cleavage on the functions of NEMO in human hepatocytes.
    Article Snippet: PHH was purchased from BioIVT and cultured in William’s Medium E (Gibco), supplemented with 1% (v/v) P-S (Gibco), 1% (v/v) NAA (Gibco), 1% (v/v) glutamax (Gibco), 0.2% (v/v) normocin (Invivogen), 2% (v/v) B27 (Gibco), 1% (v/v) N2 supplement (Gibco), 100 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 μM Y27632 (Peprotech), 1 μM A83-01 (Tocris) and cultured at 37°C with 5% CO2.

    Article Title: Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
    Article Snippet: Cells were seeded at a density of 2.7 × 10 4 cells/cm 2 in culture flasks and were cultured in William’s Medium E (Gibco/Thermo Fisher Scientific, Darmstadt, Germany) containing 10% FCS, 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 μg/mL insulin (Merck), 50 μM hydrocortisone-hemisuccinate (Biomol, Hamburg, Germany) and 1X AAS.

    Article Title: The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria.
    Article Snippet: Livers were then perfused with William’s medium E (Gibco) and 40U/L of collagenase IV (Worthington Biochemical Corporation).

    Article Title: Salsalate improves the anti-tumor efficacy of lenvatinib in MASH-driven hepatocellular carcinoma
    Article Snippet: Cells were allowed to adhere overnight, and all experiments were performed in William’s Medium E (Gibco-Thermo Fisher Scientific, Waltham, MA, USA, Cat # A12176-01) supplemented with 10% FBS (unless serum starved), 1% antibiotic–antimycotic solution, and 1% L-glutamine.

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: PHH were resuspended in cooled William’s Medium E (Gibco, Thermo Fisher Scientific, cat. no. 32551087), supplemented with 10% FBS (Sigma-Aldrich, USA, cat. no. S0615-500ml), 15 mM HEPES (cat. no. 15630–056), 1 mM sodium pyruvate (cat. no. 11360– 039), 1% Penicillin/Streptomycin (cat. no. 15140122), 1% MEM NEAA (cat. no. 11140– 035, all provided by Gibco, Thermo Fisher Scientific, USA), 1 μg/mL Dexamethasone (Jenapharm/MIBE GmbH, Germany, cat. no. PZN: 08704404), and 32 U/L human insulin (Eli Lilly, Germany, cat. no. PZN: 02526396).

    Article Title: Discovery of the Clinical Candidate S-892216 : A Second-Generation of SARS-CoV-2 3CL Protease Inhibitor for Treating COVID-19.
    Article Snippet: Regarding liver microsomes, the assays were conducted as previously reported.25 Regarding hepatocytes, the compounds were incubated with 1.0 × 106 cells/ mL in suspension in the William’s Medium E (Thermo Fisher Scientific Inc.) at 37 °C.

    Isolation:

    Article Title: METTL3 and FTO Regulate Heat Stress Response in Hu Sheep Through Lipid Metabolism via m6A Modification
    Article Snippet: For lipid deposition, primary hepatocytes were incubated with a 1.2 mM fatty acid solution [oleic acid (OA, Sigma-Aldrich): palmitic acid (PA, Sigma-Aldrich) = 2:1] in William’s Medium E (Gibco) containing 15% FBS (Gibco) for 24 h ( n = 3, three technical repetitions were performed for each sample).

    Article Title: Limited impact of hepatitis A virus 3C protease-mediated cleavage on the functions of NEMO in human hepatocytes.
    Article Snippet: PHH was purchased from BioIVT and cultured in William’s Medium E (Gibco), supplemented with 1% (v/v) P-S (Gibco), 1% (v/v) NAA (Gibco), 1% (v/v) glutamax (Gibco), 0.2% (v/v) normocin (Invivogen), 2% (v/v) B27 (Gibco), 1% (v/v) N2 supplement (Gibco), 100 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 μM Y27632 (Peprotech), 1 μM A83-01 (Tocris) and cultured at 37°C with 5% CO2.

    Article Title: Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
    Article Snippet: Cells were seeded at a density of 2.7 × 10 4 cells/cm 2 in culture flasks and were cultured in William’s Medium E (Gibco/Thermo Fisher Scientific, Darmstadt, Germany) containing 10% FCS, 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 μg/mL insulin (Merck), 50 μM hydrocortisone-hemisuccinate (Biomol, Hamburg, Germany) and 1X AAS.

    Article Title: The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria.
    Article Snippet: Livers were then perfused with William’s medium E (Gibco) and 40U/L of collagenase IV (Worthington Biochemical Corporation).

    Article Title: Salsalate improves the anti-tumor efficacy of lenvatinib in MASH-driven hepatocellular carcinoma
    Article Snippet: Cells were allowed to adhere overnight, and all experiments were performed in William’s Medium E (Gibco-Thermo Fisher Scientific, Waltham, MA, USA, Cat # A12176-01) supplemented with 10% FBS (unless serum starved), 1% antibiotic–antimycotic solution, and 1% L-glutamine.

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: PHH were resuspended in cooled William’s Medium E (Gibco, Thermo Fisher Scientific, cat. no. 32551087), supplemented with 10% FBS (Sigma-Aldrich, USA, cat. no. S0615-500ml), 15 mM HEPES (cat. no. 15630–056), 1 mM sodium pyruvate (cat. no. 11360– 039), 1% Penicillin/Streptomycin (cat. no. 15140122), 1% MEM NEAA (cat. no. 11140– 035, all provided by Gibco, Thermo Fisher Scientific, USA), 1 μg/mL Dexamethasone (Jenapharm/MIBE GmbH, Germany, cat. no. PZN: 08704404), and 32 U/L human insulin (Eli Lilly, Germany, cat. no. PZN: 02526396).

    Article Title: Discovery of the Clinical Candidate S-892216 : A Second-Generation of SARS-CoV-2 3CL Protease Inhibitor for Treating COVID-19.
    Article Snippet: Regarding liver microsomes, the assays were conducted as previously reported.25 Regarding hepatocytes, the compounds were incubated with 1.0 × 106 cells/ mL in suspension in the William’s Medium E (Thermo Fisher Scientific Inc.) at 37 °C.

    Cell Culture:

    Article Title: METTL3 and FTO Regulate Heat Stress Response in Hu Sheep Through Lipid Metabolism via m6A Modification
    Article Snippet: For lipid deposition, primary hepatocytes were incubated with a 1.2 mM fatty acid solution [oleic acid (OA, Sigma-Aldrich): palmitic acid (PA, Sigma-Aldrich) = 2:1] in William’s Medium E (Gibco) containing 15% FBS (Gibco) for 24 h ( n = 3, three technical repetitions were performed for each sample).

    Article Title: Limited impact of hepatitis A virus 3C protease-mediated cleavage on the functions of NEMO in human hepatocytes.
    Article Snippet: PHH was purchased from BioIVT and cultured in William’s Medium E (Gibco), supplemented with 1% (v/v) P-S (Gibco), 1% (v/v) NAA (Gibco), 1% (v/v) glutamax (Gibco), 0.2% (v/v) normocin (Invivogen), 2% (v/v) B27 (Gibco), 1% (v/v) N2 supplement (Gibco), 100 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 μM Y27632 (Peprotech), 1 μM A83-01 (Tocris) and cultured at 37°C with 5% CO2.

    Article Title: Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
    Article Snippet: Cells were seeded at a density of 2.7 × 10 4 cells/cm 2 in culture flasks and were cultured in William’s Medium E (Gibco/Thermo Fisher Scientific, Darmstadt, Germany) containing 10% FCS, 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 μg/mL insulin (Merck), 50 μM hydrocortisone-hemisuccinate (Biomol, Hamburg, Germany) and 1X AAS.

    Article Title: The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria.
    Article Snippet: Livers were then perfused with William’s medium E (Gibco) and 40U/L of collagenase IV (Worthington Biochemical Corporation).

    Article Title: Salsalate improves the anti-tumor efficacy of lenvatinib in MASH-driven hepatocellular carcinoma
    Article Snippet: Cells were allowed to adhere overnight, and all experiments were performed in William’s Medium E (Gibco-Thermo Fisher Scientific, Waltham, MA, USA, Cat # A12176-01) supplemented with 10% FBS (unless serum starved), 1% antibiotic–antimycotic solution, and 1% L-glutamine.

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: PHH were resuspended in cooled William’s Medium E (Gibco, Thermo Fisher Scientific, cat. no. 32551087), supplemented with 10% FBS (Sigma-Aldrich, USA, cat. no. S0615-500ml), 15 mM HEPES (cat. no. 15630–056), 1 mM sodium pyruvate (cat. no. 11360– 039), 1% Penicillin/Streptomycin (cat. no. 15140122), 1% MEM NEAA (cat. no. 11140– 035, all provided by Gibco, Thermo Fisher Scientific, USA), 1 μg/mL Dexamethasone (Jenapharm/MIBE GmbH, Germany, cat. no. PZN: 08704404), and 32 U/L human insulin (Eli Lilly, Germany, cat. no. PZN: 02526396).

    Article Title: Discovery of the Clinical Candidate S-892216 : A Second-Generation of SARS-CoV-2 3CL Protease Inhibitor for Treating COVID-19.
    Article Snippet: Regarding liver microsomes, the assays were conducted as previously reported.25 Regarding hepatocytes, the compounds were incubated with 1.0 × 106 cells/ mL in suspension in the William’s Medium E (Thermo Fisher Scientific Inc.) at 37 °C.

    Atomic Absorption Spectroscopy:

    Article Title: METTL3 and FTO Regulate Heat Stress Response in Hu Sheep Through Lipid Metabolism via m6A Modification
    Article Snippet: For lipid deposition, primary hepatocytes were incubated with a 1.2 mM fatty acid solution [oleic acid (OA, Sigma-Aldrich): palmitic acid (PA, Sigma-Aldrich) = 2:1] in William’s Medium E (Gibco) containing 15% FBS (Gibco) for 24 h ( n = 3, three technical repetitions were performed for each sample).

    Article Title: Limited impact of hepatitis A virus 3C protease-mediated cleavage on the functions of NEMO in human hepatocytes.
    Article Snippet: PHH was purchased from BioIVT and cultured in William’s Medium E (Gibco), supplemented with 1% (v/v) P-S (Gibco), 1% (v/v) NAA (Gibco), 1% (v/v) glutamax (Gibco), 0.2% (v/v) normocin (Invivogen), 2% (v/v) B27 (Gibco), 1% (v/v) N2 supplement (Gibco), 100 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 μM Y27632 (Peprotech), 1 μM A83-01 (Tocris) and cultured at 37°C with 5% CO2.

    Article Title: Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
    Article Snippet: Cells were seeded at a density of 2.7 × 10 4 cells/cm 2 in culture flasks and were cultured in William’s Medium E (Gibco/Thermo Fisher Scientific, Darmstadt, Germany) containing 10% FCS, 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 μg/mL insulin (Merck), 50 μM hydrocortisone-hemisuccinate (Biomol, Hamburg, Germany) and 1X AAS.

    Article Title: The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria.
    Article Snippet: Livers were then perfused with William’s medium E (Gibco) and 40U/L of collagenase IV (Worthington Biochemical Corporation).

    Article Title: Salsalate improves the anti-tumor efficacy of lenvatinib in MASH-driven hepatocellular carcinoma
    Article Snippet: Cells were allowed to adhere overnight, and all experiments were performed in William’s Medium E (Gibco-Thermo Fisher Scientific, Waltham, MA, USA, Cat # A12176-01) supplemented with 10% FBS (unless serum starved), 1% antibiotic–antimycotic solution, and 1% L-glutamine.

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: PHH were resuspended in cooled William’s Medium E (Gibco, Thermo Fisher Scientific, cat. no. 32551087), supplemented with 10% FBS (Sigma-Aldrich, USA, cat. no. S0615-500ml), 15 mM HEPES (cat. no. 15630–056), 1 mM sodium pyruvate (cat. no. 11360– 039), 1% Penicillin/Streptomycin (cat. no. 15140122), 1% MEM NEAA (cat. no. 11140– 035, all provided by Gibco, Thermo Fisher Scientific, USA), 1 μg/mL Dexamethasone (Jenapharm/MIBE GmbH, Germany, cat. no. PZN: 08704404), and 32 U/L human insulin (Eli Lilly, Germany, cat. no. PZN: 02526396).

    Article Title: Discovery of the Clinical Candidate S-892216 : A Second-Generation of SARS-CoV-2 3CL Protease Inhibitor for Treating COVID-19.
    Article Snippet: Regarding liver microsomes, the assays were conducted as previously reported.25 Regarding hepatocytes, the compounds were incubated with 1.0 × 106 cells/ mL in suspension in the William’s Medium E (Thermo Fisher Scientific Inc.) at 37 °C.

    Membrane:

    Article Title: METTL3 and FTO Regulate Heat Stress Response in Hu Sheep Through Lipid Metabolism via m6A Modification
    Article Snippet: For lipid deposition, primary hepatocytes were incubated with a 1.2 mM fatty acid solution [oleic acid (OA, Sigma-Aldrich): palmitic acid (PA, Sigma-Aldrich) = 2:1] in William’s Medium E (Gibco) containing 15% FBS (Gibco) for 24 h ( n = 3, three technical repetitions were performed for each sample).

    Article Title: Limited impact of hepatitis A virus 3C protease-mediated cleavage on the functions of NEMO in human hepatocytes.
    Article Snippet: PHH was purchased from BioIVT and cultured in William’s Medium E (Gibco), supplemented with 1% (v/v) P-S (Gibco), 1% (v/v) NAA (Gibco), 1% (v/v) glutamax (Gibco), 0.2% (v/v) normocin (Invivogen), 2% (v/v) B27 (Gibco), 1% (v/v) N2 supplement (Gibco), 100 mM nicotinamide (Sigma-Aldrich), 1.25 mM N-acetylcysteine (Sigma-Aldrich), 10 μM Y27632 (Peprotech), 1 μM A83-01 (Tocris) and cultured at 37°C with 5% CO2.

    Article Title: Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
    Article Snippet: Cells were seeded at a density of 2.7 × 10 4 cells/cm 2 in culture flasks and were cultured in William’s Medium E (Gibco/Thermo Fisher Scientific, Darmstadt, Germany) containing 10% FCS, 2 mM L-glutamine (Gibco/Thermo Fisher Scientific), 5 μg/mL insulin (Merck), 50 μM hydrocortisone-hemisuccinate (Biomol, Hamburg, Germany) and 1X AAS.

    Article Title: The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria.
    Article Snippet: Livers were then perfused with William’s medium E (Gibco) and 40U/L of collagenase IV (Worthington Biochemical Corporation).

    Article Title: Salsalate improves the anti-tumor efficacy of lenvatinib in MASH-driven hepatocellular carcinoma
    Article Snippet: Cells were allowed to adhere overnight, and all experiments were performed in William’s Medium E (Gibco-Thermo Fisher Scientific, Waltham, MA, USA, Cat # A12176-01) supplemented with 10% FBS (unless serum starved), 1% antibiotic–antimycotic solution, and 1% L-glutamine.

    Article Title: CD271 sorting for improved liver cell isolation: Semiautomated and simultaneous preparation of parenchymal and non-parenchymal cells from mouse and human livers
    Article Snippet: PHH were resuspended in cooled William’s Medium E (Gibco, Thermo Fisher Scientific, cat. no. 32551087), supplemented with 10% FBS (Sigma-Aldrich, USA, cat. no. S0615-500ml), 15 mM HEPES (cat. no. 15630–056), 1 mM sodium pyruvate (cat. no. 11360– 039), 1% Penicillin/Streptomycin (cat. no. 15140122), 1% MEM NEAA (cat. no. 11140– 035, all provided by Gibco, Thermo Fisher Scientific, USA), 1 μg/mL Dexamethasone (Jenapharm/MIBE GmbH, Germany, cat. no. PZN: 08704404), and 32 U/L human insulin (Eli Lilly, Germany, cat. no. PZN: 02526396).

    Article Title: Discovery of the Clinical Candidate S-892216 : A Second-Generation of SARS-CoV-2 3CL Protease Inhibitor for Treating COVID-19.
    Article Snippet: Regarding liver microsomes, the assays were conducted as previously reported.25 Regarding hepatocytes, the compounds were incubated with 1.0 × 106 cells/ mL in suspension in the William’s Medium E (Thermo Fisher Scientific Inc.) at 37 °C.



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